Biological Sciences, Lehigh University Lehigh University home page Department of Biological Sciences home page
Faculty, Biological Sciences, Lehigh University

Krystle McLaughlin, Ph.D.
Professor of Practice

Research Interest:
Structural Biophysics

Iacocca Hall
111 Research Drive, B218
Bethlehem, PA 18015


email Dr. McLaughlin

Read about Dr. McLaughlin's work
in the Spring 2015 issue of Acumen, a publication of Lehigh University's
College of Arts and Sciences
Welcome to the McLaughlin Lab!

Krystle McLaughlin, Ph.D.Researchers in the McLaughlin Lab use the tools of structural biology to determine the molecular structure of proteins and protein complexes. Structural biology coupled with molecular biology, biochemical, and biophysical techniques allow us to investigate structure-function relationship in various systems.

The McLaughlin Lab has multiple research projects in progress.

A major research interest of our lab is the the study and characterization of protein targets from the commensal human gut bacteria Bacteroides ovatus. Gut microbiota populations in healthy individuals show marked differences compared with populations in individuals with autoimmune diseases. An overabundance of the normally commensal gut microbe Bacteriodes ovatus has been observed in the autoimmune disorders ulcerative colitis, Crohn’s disease, type I diabetes and celiac disease. We will use x-ray crystallography coupled with several biochemical and biophysical techniques to study the microbe Bacteriodes ovatus. We are performing functional and structural investigations of protein targets from B. ovatus hypothesized to play a part in provoking a human autoimmune response or on strain survival.

Krystle McLaughlin, Ph.D.

The other focus of the lab involves a collaboration with Dr. Vassie Ware and the Lehigh SEA-PHAGES program. We are structurally characterizing novel protein targets from bacteriophages identified by Lehigh undergraduates students in the Lehigh in the S.E.A course.

Our primary research tool is x-ray crystallography, and we also employ biochemical and biophysical methods such as recombinant protein purification, fluorescence anisotropy and enzyme-linked assays to study various protein targets.

Krystle McLaughlin, Ph.D.


Agrawal A., McLaughlin K.J., Jenkins J.L., Kielkopf C.L. “A Structure-Guided U2AF65 Variant Improves Recognition and Splicing of a Defective pre-mRNA.” Proc Natl Acad Sci U S A. 2014, 111(49):17420-5 

McLaughlin K.J., Nash R.P., Redinbo M.R. “Unique Helicase Determinants in the essential conjugative factor TraI from Salmonella typhimurium plasmid pCU1.”  J. Bacteriol. 2014, 196(17):3082-90 doi:10.1128/JB.01496-14  Pubmed

McLaughlin K.J., Jenkins J.L., Kielkopf C.L. “Large Favorable Enthalpy Changes Drive Specific RNA Recognition by RNA Recognition Motif Proteins.” Biochemistry 2011, 50(9):1429-31 Text/PDF

McLaughlin K.J., Strain-Damerell, C.M., Xie, K., Brekasis, D., Soares, A.S., Paget, M.S. and Kielkopf, C.L. Structural Basis for NADH/NAD+ Redox Sensing by a Rex-Family Repressor. Molecular Cell. 2010 May 28;38(4):563-75.  Text/PDF

Buboltz JT, Bwalya C, Williams K, Schutzer M. “High-resolution mapping of phase behavior in a ternary lipid mixture: do lipid-raft phase boundaries depend on the sample preparation procedure?” Langmuir 2007, 23:11968-71. Text/PDF

In the News | Links

Krystle McLaughlin, Ph.D.

  • "Structures of Phages"- Acumen, Lehigh University College of Arts and Sciences magazine. April 2015

Biological Sciences
111 Research Drive
Bethlehem, PA 18015
Phone: 610-758-3680
Fax: 610-758-4004