Undergraduate Program in Biological Sciences
  • Fluorescence Microscopes Fluorescence Microscopes
  • Laser Microdissection
  • Transmission Electron Microscope Transmission Electron Microscope
  • Confocal Microscope Confocal Microscope
  • Nikon Swept Field Confocal Nikon Swept Field Confocal
  • Nikon Biostation Nikon Biostation
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Microscopy

The Department of Biological Sciences is well-equipped with a variety of high-end microscope facilities.

Fluorescence Microscopy
Confocal Microscopy
Transmission Electron Microscopy
Laser Microdissection

 

Fluorescence Microscopy

  • Nikon TE-2000U with phase contrast
  • Nikon TE-2000U with DIC
  • Nikon Eclipse E800
  • Nikon E1000
  • Nikon SMZ1500 stereomicroscope
  • Nikon Biostation IM

The department maintains a variety of inverted and upright microscopes capable of fluorescent, DIC and phase contrast imaging. All microscopes have designated cameras and software for capturing high-quality images. The Biostation IM is an incubated, CO2-controlled, enclosed microscope that allows for multi-day time-lapse imaging of live cells.

 

Confocal Microscopy

  • Zeiss LSM880 scanning confocal
  • Nikon LiveScan Swept Field confocal

The Department of Biological Sciences has two confocal microscopes – a Zeiss LSM880 scanning microscope and a Nikon Swept Field. The Zeiss is equipped with lasers for excitation at 405, 458, 488, 501, 514, 543, and 633nm. A heated stage is also available for live cell imaging, although users must provide their own chamber. The Nikon Swept Field is
designed specifically for live-cell imaging and can excite at 440, 488, and 561nm.

 

Transmission Electron Microscopy

  • Delong Instruments LVEM5 benchtop TEM

The LVEM5 is a unique benchtop TEM that operates at only 5keV. This reduced voltage is suitable for imaging of samples that can be deposited out of solution, or are less than 50nm in thickness. We use this microscope extensively for our SEA Phages program

 

Laser Microdissection

  • Molecular Machines MMI CellCut laser microdissection

The MMI CellCut laser microdissection system allow for precise, user-defined sections of material to be cut and removed from special slides or dishes. The instrument is then able to transfer these sections to a tube so they can be further analyzed. Our system utilizes a Nikon TE-2000S inverted fluorescent microscope.

 

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Biological Sciences
111 Research Drive
Bethlehem, PA 18015
Phone: 610-758-3680
Fax: 610-758-4004
Email: inbios@lehigh.edu


©2015