Research
Interests:
Our
broad interests lie in addressing the fundamental questions surrounding
the interactions of lipid bilayers with proteins. Using various
biophysical techniques namely NMR and analytical ultracentrifugation,
we aim to understand the behavior of proteins in the lipid milieu
on a molecular level. Our research capitalizes on the use of bicelles,
a "native-like" membrane mimic which has great potential to overcome
some of the hurdles that have limited the study of membrane proteins.
Teaching
Interests:
Biochemistry
and biophysical chemistry.
Recent
Publications:
Glover,
K. J.; Weerapana, E.; Chen, M. M.; Imperiali, B., Direct biochemical
evidence for the utilization of UDP-bacillosamine by PglC, an essential
glycosyl-1-phosphate transferase in the Campylobacter jejuni N-linked
glycosylation pathway. Biochemistry 2006 ,
45, (16), 5343-5350.
Glover,
K. J.; Weerapana, E.; Numao, S.; Imperiali, B., Chemo-Enzymatic
Synthesis of Glycopeptides Using PglB, a Bacterial Oligosaccharyl
Transferase from Campylobacter Jejuni . Chem. Biol.
2005 , 12, (12), 1311-1315.
Weerapana,
E.; Glover, K. J.; Chen, M. M.; Imperiali, B., Investigating Bacterial
N -Linked Glycosylation: Synthesis and Glycosyl Acceptor
Activity of the Undecaprenylpyrophosphate-Linked Bacillosamine.
J. Am. Chem. Soc. 2005 , 127, (40), 13766-13767.
Glover,
K. J.; Weerapana, E.; Imperiali, B., In Vitro Assembly of the Undecaprenylpyrophosphate-Linked
Heptasaccharide for Prokaryotic N -Linked Glycosylation.
Proc. Natl. Acad. Sci. U.S.A. 2005 , 102,
(40), 14255-14259.
Whiles,
J. A.; Glover, K. J.; Vold, R. R.; Komives, E. A., Methods for Studying
Transmembrane Peptides in Bicelles: Consequences of Hydrophobic
Mismatch and Peptide Sequence. J. Magn. Reson. 2002,
158, (1-2), 149-156.
Glover,
K. J.; Whiles, J. A.; Vold, R. R.; Melacini, G., Position of Residues
in Transmembrane Peptides with Respect to the Lipid Bilayer: A Combined
Lipid NOEs and Water Chemical Exchange Approach in Phospholipid
Bicelles. J. Biomol. NMR 2002, 22, (1),
57-64.
Whiles,
J. A.; Brasseur, R.; Glover, K. J.; Melacini, G.; Komives, E. A.;
Vold, R. R., Orientation and Effects of Mastoparan X on Phospholipid
Bicelles. Biophys. J. 2001, 80, (1),
280-293.
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