3D Printing with Peptide-Polymer Conjugates to Regenerate the Osteochondral Interface
Department: Computer Science / IDEAS
Advisor: Mooi Choo Chuah
The osteochondral interface refers to the interface between cartilage and bone, two mechanically and compositionally dissimilar tissues. Damage to this interface is caused by disruption to cartilage, an avascular tissue that has limited ability to self-repair. This can lead to significant pain and immobility that eventually results in total knee replacement, which often requires costly revision surgeries. Tissue engineering offers strategies to prevent or delay this by fabricating biomaterials mimic the body’s native tissues to guide tissue regeneration. Notably, the native extracellular matrix (ECM) in the osteochondral interface is composed of different biomolecules arranged in a complex and hierarchical organization to yield specific functions. To achieve this, we developed a peptide-polymer functionalization approach to control the spatial arrangement of multiple peptides within a single scaffold. Hyaluronic acid (HA)-binding and mineralizing peptides designed to promote human mesenchymal stem cell (hMSC) chondrogenesis and osteogenesis, respectively, were conjugated to poly(caprolactone) (PCL) to form peptide-PCL conjugates. These conjugates were then added to PCL-based inks and 3D printed to control the spatial deposition of each peptide-PCL conjugate. This technique allowed us to organize HA-binding and mineralizing peptides on opposing sides of the same scaffold. The scaffolds were sectioned and labeled with specific fluorophores to visualize the spatial distribution of peptides using confocal microscopy. Future and ongoing work involves in vitro experiments to investigate spatial hMSC differentiation and matrix formation.
About Divya Patel:
Divya is from Towanda, PA and is currently a senior at Lehigh University majoring in Bioengineering, with a focus in Biomechanics and Biomaterials. She is the vice president of Lehigh’s Biomedical Engineering Society, and also plays an active role in other clubs including the Happiness Collective, Indian Students Association, and the Society of Woman Engineers. Divya has attended LeaderShape® and the Great Pocono Escape in order to develop her leadership skills, and for two years, Divya has been part of the Office of First-Year Experience staff as a Camp Hawk Counselor. Divya had the unfortunate luck of tearing her meniscus as well as both of her ACLs due to sports-related injuries in high school, and as a result, she has become very passionate about the field of tissue engineering. This passion has led Divya to do research with Dr. Lesley Chow, whose work involves tissue-engineering biomaterials. Divya will be graduating in May 2018, after which she will intern at Nihon Kohden.